Abstract: OBJECTIVE To explore the epidemiological characteristics and drug resistance mechanisms of type ST2735 NDM-5-producing carbapenem-resistant Klebsiella pneumoniae (CRKP) isolated from children. METHODS Totally 24 strains of imipenem-resistant Klebsiella pneumoniae were initially isolated from the children who were treated in the Affiliated Hospital of Xuzhou Medical University from Mar 2018 to Mar 2022. Carbapenemases were detected by means of modified carbapenem inactivation method (mCIM) and ethylenediamine tetraacetic acid-carbapenem inactivation method (eCIM); carbapenemase genes were detected by polymerase chain reaction (PCR), and the homology was detected by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). RESULTS A total of 24 strains of imipenem-resistant K.pneumoniae were isolated from the children between Mar 2018 and Mar 2022, 15 (62.50%) of which were type NDM-5 CRKP, and the 15 children had underlying diseases and history of antibiotic therapy. All of the 15 strains of type NDM-5 CRKP were resistant to cephalosporins, carbapenems and ceftazidime-averebatam. MLST showed that ST2735 was dominant, accounting for 25.00%. PFGE analysis indicated that 6 strains of ST2735 type CRKP were highly homologous and were isolated from neonatal intensive care unit (NICU). CONCLUSION There is a clonal prevalence of ST2735 type NDM-5-producing CRKP in the NICU. It is necessary for the hospital to strengthen the surveillance and prevention of nosocomial infection so as to prevent novel prevalent hazards.