Abstract: OBJECTIVE To understand the prevalence of ventilator-associated pneumonia (VAP) in Suzhou in recent three years and investigate the distribution and drug resistance of pathogens. METHODS From Jan 2020 to Dec 2022, the monitoring data of indexes for quality control of nosocomial infection that were reported from secondary or secondary above medical institutions were recruited as the research subjects. The monitoring data of VAP from 51 hospitals were statistically analyzed. RESULTS A total of 1 903 case-times(4.31‰) of patients had VAP from 2020 to 2022, the case-time infection rate was higher in the tertiary hospitals than in the secondary hospitals, and the case-time infection rate was higher in the tertiary general hospitals than in the specialized hospitals (P<0.05). There was no significant difference in the morbidity rate of VAP between the secondary general hospitals and the specialized hospitals. The incidence of VAP showed a downward trend in the recent three years (P<0.05). Among the pathogens isolated from the VAP patients, gram-negative bacteria were dominant, accounting for 82.08%; Klebsiella pneumoniae, Acinetobacter baumannii and Pseudomonas aeruginosa were the predominant species; the isolation rates of carbapenem-resistant K.pneumoniae (CRKP), carbapenem-resistant A.baumannii (CRAB) , carbapenem-resistant P.aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA) were 26.97%, 74.58%, 32.58% and 52.69%, respectively. The constituent ratio of CRAB was the highest among the MDROs, followed by CRKP, CRPA and MRSA. The drug resistance rate of the K.pneumoniae strains to most of the antibiotics was less than 40%; the CRKP strains were highly drug-resistant. The A.baumannii and CRAB strains were highly susceptible to polymyxin, tigecycline and minocycline. P.aeruginosa, CRPA, S.aureus and MRSA strains were highly susceptible to most of the antibiotics. The drug resistance rate of Stenotrophomonas maltophilia to ceftazidime was 39.17%; the drug resistance rate of Burkholderia cepacia to cefoperazone-sulbactam and ticarcillin-clavulanic acid was more than 70%. CONCLUSION There is certain difference in the epidemiological characteristics of VAP among the various types of hospitals in the city. The infection can be effectively controlled and the transmission of drug-resistant strains can be slowed down by combining with the monitoring data.