儿童肺炎支原体耐药性及血清miR-126、miR-155、LncRNA GAS5表达对肺炎病情程度的预测价值

Drug resistance of children with Mycoplasma pneumoniae infection and values of serum miR-126, miR-155 and LncRNA GAS5 in prediction of illness condition

    摘要
  • 摘要:
    目的 探讨儿童肺炎支原体耐药性及血清微小核糖核酸(miR)-126、miR-155、长链非编码RNA生长抑制特异因子5(LncRNA GAS5)表达对肺炎病情程度的预测价值。
    方法 选取2022年1月-2023年9月南通市妇幼保健院收治的82例肺炎支原体感染患儿为病例组，随机选取98名同期正常健康体检儿童为对照组，病例组根据病情程度分为轻度组57例和中重度组25例，分析肺炎支原体耐药性，比较病例组和对照组、中重度组和轻度组血清miR-126、miR-155、LncRNA GAS5水平，并分析其预测价值。
    结果 82株肺炎支原体对阿奇霉素、乙酰螺旋霉素耐药性较高，对克林霉素敏感性较高。血清miR-126、miR-155水平病例组高于对照组(P < 0.05)，中重度组高于轻度组(P < 0.05)，LncRNA GAS5水平病例组低于对照组(P < 0.05)，中重度组低于轻度组(P < 0.05)。血清miR-126、miR-155、LncRNA GAS5水平联合检测对肺炎支原体感染患儿病情严重程度的诊断曲线下面积(AUC)值高于单独检测(P < 0.05)，且联合检测的敏感度、特异度分别为84.00%和94.74%。
    结论 肺炎支原体感染患儿对阿奇霉素、乙酰螺旋霉素相对较耐药，血清miR-126、miR-155水平呈高表达，LncRNA GAS5水平呈低表达，三指标表达水平变化与肺炎支原体感染患儿病情严重程度关系密切，三指标联合检测对肺炎支原体感染患儿病情严重程度预测价值较高。

     

    Abstract:
    OBJECTIVE To explore the drug resistance of the children with Mycoplasma pneumoniae pneumonia and analyze the values of serum micro ribonucleic acid (miR)-126, miR-155 and long non-coding RNA growth inhibition factor 5 (LncRNA GAS5) in prediction of the severity of diseases.
    METHODS A total of 82 children with M.pneumoniae infection who were treated in Nantong Maternal and Child Health Hospital from Jan. 2022 to Sep. 2023 were assigned as the case group, meanwhile, 98 healthy children who received physical examination were chosen as the control group. The children of the case group were divided into the mild group with 57 cases and the moderate and severe group with 25 cases according to the severity of disease. The drug resistance of M.pneumoniae was analyzed, the levels of serum miR-126, miR-155 and LncRNA GAS5 were compared among the case group, the control group, the moderate and severe group and the mild group. The predictive values of the indexes were analyzed.
    RESULTS The drug resistance rates of the 82 strains of M.pneumoniae to azithromycin and acetylspiramycin were higher. The levels of serum miR-126 and miR-155 of the case group were higher than those of the control group(P < 0.05), the levels of the above indexes of the moderate and severe group were higher than those of the mild group(P < 0.05); the LncRNA GAS5 level of the case group was lower than that of the control group(P < 0.05), and the level of the above index of the moderate and severe group was lower than that of the mild group(P < 0.05). The area under curve (AUC) of the joint detection of serum miR-126, miR-155 and LncRNA GAS5 was higher than that of the single detection in diagnosis of the severity of disease of the children with M.pneumoniae infection(P < 0.05), and the sensitivity of the joint detection was 84.00%, the specificity 94.74%.
    CONCLUSIONS The children with M.pneumoniae infection are relatively resistant to azithromycin and acetylspiramycin and show the high expressions of serum miR-126, mirR-155 and the low expression of LncRNA GAS5. The change of expressions of three indexes is closely associated with the severity of disease of the children with M.pneumoniae infection. The joint detection of the three indexes has high value in prediction of the severity of disease of the children with M.pneumoniae.

     

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