Abstract:
OBJECTIVE To investigate the causes for one incident of outbreak of nosocomial infection induced by Mycobacterium abscessus and verify the effectiveness of prevention measures so as to provide scientific bases for effective control of the nosocomial infection induced by M.abscessus.
METHODS Totally 4 patients who were found to be with suspected pulmonary tuberculosis in the same ward of Tianyou Hospital of Putuo District from Sep. 27, 2021 to Oct. 1, 2021 were enrolled in the study. The epidemiological survey, screening of close contact, clinical laboratory test and health education were carried out for the enrolled patients. Totally 32 environmental samples were collected for isolation and culture of pathogens, metagenomic next-generation sequencing (mNGS) was performed, and the disinfection was carried out under guidance while the epidemic was treated.
RESULTS The sputum smear test, culture of Mycobacterium, GeneXpert test and mNGS were performed for the 4 patients with suspected pulmonary tuberculosis. Among the 4 patients, 3 were positive for culture of M.abscessus. The mNGS showed that all of the 4 patients were positive for sequencing of M.abscessus. Totally 32 environmental samples from different sources showed negative for mNGS of M.abscessus. There were 16 cases of close contacts who received screening of symptoms, chest imaging examination, sputum test and one-year follow-up, and all of the results were negative. There was no report of consecutive infection case during the one-year follow-up after a series of infection control measures such as the disinfection of ventilation facilities, ventilator pipelines and bronchoscopes were carried out.
CONCLUSIONS The outbreak of incident of nosocomial infection is caused by M.abscessus, which is more likely isolated from environment. mNGS can capture special pathogens and play an important role in identifying the outbreak of nosocomial infection.
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