低温等离子体对大鼠铜绿假单胞菌感染创面的修复作用

Efficacy of low temperature plasma in treatment of rats with Pseudomonas aeruginosa wound infection

    摘要
  • 摘要:
    目的 探究低温等离子体(LTP) 治疗大鼠铜绿假单胞菌(PA) 感染创面的有效性。
    方法 采用20只SD大鼠建立PA感染创面, 随机分为LTP组(n =10) 和空气对照组(n =10)。观察愈合过程中创面愈合动力学、细菌载荷量、组织病理学、细胞因子水平及微环境特征等变化。
    结果 LTP加速了PA感染创面的愈合, 治疗3 d后创面面积减小, 炎症反应减轻; 7 d后感染症状消退, 创面干燥结痂; 15 d后创面缩小, 愈合趋势良好。重复测量方差分析表明, LTP对创面覆盖率影响有统计学意义(F时间=10.230, P < 0.001; F组间=11.340, P =0.001; F交互=2.890, P =0.042); 细菌载荷定量显示: LTP组在治疗3、7、15 d时细菌载荷量低于对照组(P < 0.05); 组织病理学结果显示: LTP组创面炎症细胞浸润减少, 胶原纤维排列整齐, 新生血管和肉芽组织生成明显。此外, LTP治疗后创面组织的pH值和活性氧(ROS) 水平改善, 且与愈合进程相匹配; 细胞因子水平检测表明: LTP能够上调VEGF和TGF-β1水平, 同时下调IL-1β和TNF-α水平, 促进创面愈合, 差异均有统计学意义(P < 0.05)。
    结论 LTP能够通过减少创面细菌数量控制感染、建立组织愈合有效微环境, 调节愈合相关细胞因子释放加速PA感染创面愈合。

     

    Abstract:
    OBJECTIVE To explore the efficacy of low temperature plasma (LTP) in treatment of the rats with Pseudomonas aeruginosa (PA) wound infection.
    METHODS Totally 20 SD rats were assigned to establish the PA wound infection models and were randomly divided into the LTP group with 10 rats and the air control group with 10 rats. The wound healing dynamics, bacterial loading capacity, histopathology, levels of cytokines and microenvironment features were observed during the healing process.
    RESULTS LTP accelerates the healing of PA infection wound, the wound area reduced after the treatment for 3 days, and the inflammatory reactions alleviated; the infection symptoms subsided after the treatment for 7 days, and the wounds became dry and scabbed; the wounds reduced in size by day 15, showing favorable healing trends. Repeated measurement analysis of variance (ANOVA) indicated that there was significant difference in the impact of LTP on the covering rate of wounds(Ftime=10.230, P < 0.001;Fbetween-group=11.340, P=0.001;Finteraction=2.890, P=0.042). The result of quantitative bacterial loads analysis showed that the bacterial loads of the LTP group were lower than those of the control group after the treatment for 3, 7 and 15 days(P < 0.05). The result of histopathology indicated that the infiltrated wound inflammatory cells of the LTP group reduced, the collagen fibers were well-aligned, and the neovascularization and formation of granulation tissues were evident. In addition, the pH value of wound tissues and reactive oxygen species (ROS) level were improved after the treatment with LTP, which was matched with the process of wound healing. The result of cytokines test showed that LTP could upregulate the levels of VEGF and TGF-β1, downregulate the levels of IL-1β and TNF-α, and promote the wound healing, with statistically significant differences observed (P < 0.05).
    CONCLUSION LTP may facilitate the control of infections by reducing the bacterial colony counts, establish the effective microenvironment for tissue healing, and accelerate the healing of wounds with PA infection by regulating the release of healing-related cytokines.

     

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