HPV感染宫颈癌组织miR-199、miR-362-3p、miR-195水平与临床分期、分化程度及淋巴结转移的关系

Relationship between miR-199, miR-362-3p and miR-195 and clinical stages, differentiation degrees and lymph node metastasis in cervical cancer patients with HPV infection

    摘要
  • 摘要:
    目的 探究人乳头状瘤病毒(HPV)感染宫颈癌患者微小核糖核酸(miR)-199、miR-362-3p、miR-195与临床分期、分化程度及淋巴结转移的关系，为宫颈癌患者HPV感染的早期预防提供参考。
    方法 选取武汉市第八医院(武汉市肛肠医院)2021年7月-2024年1月收治的169例HPV感染宫颈癌患者为阳性组，HPV阴性宫颈癌患者175例为阴性组，同时选取同期宫颈良性疾病患者187例为对照组；比较三组及不同临床分期、不同分化程度、有无淋巴结转移HPV感染宫颈癌患者miR-199、miR-362-3p、miR-195水平差异，利用Spearman分析miR-199、miR-362-3p、miR-195水平与临床分期、分化程度、淋巴结转移的相关性。
    结果 阳性组miR-199、miR-362-3p、miR-195水平分别为(0.83±0.22)、(1.39±0.34)、(0.74±0.20)，低于阴性组和对照组，且阴性组低于对照组(P < 0.05)；Ⅲ~Ⅳ期HPV感染宫颈癌患者miR-199、miR-362-3p、miR-195水平低于Ⅱ期、Ⅰ期，且Ⅱ期低于Ⅰ期(P < 0.05)；低分化HPV感染宫颈癌患者miR-199、miR-362-3p、miR-195水平低于中高分化(P < 0.05)；有淋巴结转移HPV感染宫颈癌患者miR-199、miR-362-3p、miR-195水平低于无淋巴结转移患者(P < 0.05)；miR-199、miR-362-3p、miR-195水平与临床分期、淋巴结转移呈负相关(r=-0.657、-0.704、-0.715及-0.685、-0.752、-0.746，P < 0.05)，三者与分化程度则呈正相关(r=0.699、0.744、0.765，P < 0.05)。
    结论 宫颈组织miR-199、miR-362-3p、miR-195在HPV感染宫颈癌患者中低表达，且三者表达与HPV感染宫颈癌患者临床分期、分化程度、淋巴结转移有关。

     

    Abstract:
    OBJECTIVE To explore the relationships between micro ribonucleic acid (miR)-199, miR-362-3p and miR-195 and clinical stages, degrees of morphologic differentiation and lymph node metastasis in cervical cancer patients with human papillomavirus (HPV) infection, so as to provide references for early prevention.
    METHODS A total of 169 cervical cancer patients with HPV-infection admitted to Wuhan Eighth Hospital (Wuhan Colorectal Hospital) from Jul. 2021 to Jan. 2024 were selected as the positive group, 175 without HPV as the negative group, and another 187 with benign cervical diseases in the same period were as the control group. The levels of miR-199, miR-362-3p and miR-195 were compared among the three groups, and among different clinical stages, degrees of morphologic differentiation and conditions of lymph node metastatic in the positive group. Spearman measure was used to analyze the correlations.
    RESULTS miR-199, miR-362-3p and miR-195 levels in the positive group were (0.83±0.22), (1.39±0.34) and (0.74±0.20), respectively, which were the lowest in the three groups, and followed by the negative and control group (P < 0.05); miR-199, miR-362-3p and miR-195 levels in stage Ⅲ-Ⅳ of cancer were the lowest in the three stages and followed by those in stage Ⅱ and stage Ⅰ (P < 0.05); their levels in patients with low-differentiated cancer were lower than those in medium/high differentiated cancer (P < 0.05); and levels of three indicators in cervical cancer patients with lymph node metastasis were lower than those in patients without lymph node metastasis (P < 0.05). miR-199, miR-362-3p and miR-195 were negatively correlated with clinical stage progression and lymph node metastasis (r=-0.657, -0.704, -0.715 and -0.685, -0.752, -0.746, respectively, all P < 0.05), and positively correlated with the degree of morphologic differentiation (r=0.699, 0.744, 0.765, respectively, all P < 0.05).
    CONCLUSION miR-199, miR-362-3p and miR-195 express lowly in the cervical tissues of HPV-infected cervical cancer patients and their levels are related to the progression of clinical stages, morphologic differentiation degrees and lymph node metastasis.

     

    • HTML全文
    • 参考文献(20)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回
下载中心