Abstract: Hypochlorous acid disinfectant has been widely noticed and applied in the medical field due to its powerful disinfecting and sterilizing ability and environmental friendly characteristics.Effective concentration of hypochlorous acid disinfectant can not only efficiently kill bacteria, viruses and other microorganisms, but also has the advantages of low toxicity, no residue and low corrosion, which is suitable for all kinds of complicated equipment and complex medical scenarios. It can be used for environmental disinfection in major medical places such as pediatric wards and hemodialysis rooms. In addition, it demonstrates unique advantages in the oral care, wound treatment, and surgical site disinfection. This article explores the properties of hypochlorous acid disinfectant to provide reference for the reasonable application in healthcare facilities. With the continuous progress of cleaning and disinfection technology and in-depth expansion of application, the prospect of hypochlorous acid disinfectant application in the medical field will be broader.