OBJECTIVE To analyze the incidence of perforation of disinfectant-sterilized gloves used for continuous blood collection in clinical laboratories and identify its risk factors.

METHODS A total of 1 198 disinfectant-sterilized gloves used for continuous blood collection in the clinical laboratory of Zhuji Sixth People′s Hospital, Zhejiang Province, from Jul. 2023 to Dec. 2023 were included. Perforation rates of the gloves were recorded, and multivariate logistic regression was performed to conclude the risk factors for perforation of disinfectant-sterilized gloves used for continuous blood collection.

RESULTS Among the 1 198 disinfectant-sterilized gloves used for continuous blood collection in the clinical laboratory, 150 exhibited perforations, with a perforation rate of 12.52%. Significant differences in perforation rates were observed based on glove continuous usage time (20.09% vs. 10.87%), disinfection frequency (15.99% vs. 9.18%), phlebotomist′s professional title (14.88% vs. 10.92%), blood donor population (17.19% vs. 11.63%), and glove size fit (11.22% vs. 20.73%) (P < 0.05). Risk factors for perforation of disinfectant-sterilized gloves used for continuous blood collection in the clinical laboratory included glove continuous usage time >60 min (OR=2.754, 95%CI: 1.423-5.333, P=0.003), disinfection frequency >6 times (OR=4.272, 95%CI: 2.287-7.979, P < 0.001), and ill-fitting glove size (OR=5.107, 95%CI: 2.899-8.995, P < 0.001).

CONCLUSIONS The perforation rate of disinfectant-sterilized gloves used for continuous blood collection remains concern. Phlebotomists in the clinical laboratory should wear properly sized sterile rubber gloves for blood collection, and replace them after >60 min of continuous use or >6 disinfection frequency.