OBJECTIVE To understand the distribution and drug resistance of multidrug-resistant organisms (MDROs) for children hospitalized in a three-A children′s hospital in Henan Province, and to provide references for clinical rationalization of drug management.

METHODS Detection of pathogens and drug sensitivity tests were performed on samples of hospitalized children from a three-A children′s hospital from Jan. 1, 2019 to Dec. 31, 2023, to analyze the distribution and drug resistance of MDRO.

RESULTS During the five years, totally 5243 strains of MDRO were isolated, of which 1674 strains were gram-positive (31.9%), mainly were methicillin-resistant Staphylococcus aureus (MRSA) (n=588), methicillin-resistant coagulase-negative human Staphylococci (MRCNS) (n=327) and methicillin-resistant Staphylococcus epidermidis (MRSE) (n=257); totally 3569 strains were gram-negative (68.1%), mainly were extended-spectrum beta-lactamase-producing Escherichia coli (n=1654), carbapenem-resistant Klebsiella pneumoniae (CRKP) (n=723) and carbapenem-resistant Acinetobacter baumannii (CRAB) (n=448). The main sources of strains were from sputa (n=2348, 44.8%), whole venous blood (whole venous blood, catheter blood, bone marrow fluid, etc.) (n=863, 16.5%), secretions (eye, wound, vaginal secretions, etc.) (n=651, 12.4%), alveolar lavage fluid (n=390, 7.4%), ascites and others(n=343, 6.5%), midstream urine(n=339, 6.5%), and others (catheter terminal, excrement etc.) (n=309, 5.9%). Extended-spectrum beta-lactamase-producing E. coli, CRKP and MRSA were the main popular MDRO strains in hospitalized children.

CONCLUSION Multi-drug resistant bacteria isolated in this hospital during the five years are mainly gram-negative bacteria, extended-spectrum beta-lactamase-producing E. coli and CRKP, suggesting that monitoring drug-resistant bacteria and supervising antibacterial drug should be strengthened.