宏基因二代测序技术对儿童肺炎支原体肺炎的诊断价值

Diagnostic value of metagenomic next generation sequencing technology for children with Mycoplasma pneumoniae pneumonia

    摘要
  • 摘要:
    目的 探讨宏基因二代基因测序(mNGS)在肺炎支原体肺炎病原体检测中的应用价值。
    方法 对蚌埠市第一人民医院2023年1-12月期间住院治疗的120例肺炎支原体肺炎患儿进行回顾性分析, 收集患者临床资料, 采集患者支气管肺泡灌洗液(BALF)、血液样本等进行mNGS检测和传统病原体检测, 比较两种不同检测方法对病原体检测的阳性率、病原学分布情况。
    结果 肺炎支原体肺炎患儿中共检出223株病原体。mNGS共检出218株病原体, 其中细菌46株, 肺炎支原体120株, 病毒51株, 真菌1株。传统方法学共检出125株, 其中细菌7株, 病毒27株, 肺炎支原体91株。mNGS方法在细菌、病毒、肺炎支原体的检出率高于传统方法学, 差异具有统计学意义(P＜0.05)。
    结论 临床中肺炎支原体肺炎儿童以混合型感染为主, 相比于繁琐的传统病原体检测, mNGS检测耗时更少、诊断效能更高。

     

    Abstract:
    OBJECTIVE To investigate the application value of metagenomic next generation sequencing (mNGS) technology in the diagnosis and treatment of Mycoplasma pneumoniae pneumonia.
    METHODS Retrospective analysis was conducted on 120 children diagnosed with M. pneumoniae pneumonia and hospitalized at the First People′s Hospital of Bengbu City from Jan. to Dec. 2023. Clinical data, bronchoalveolar lavage fluid (BALF), and blood samples were collected for mNGS and traditional pathogenic detection method, the positive rates and etiological distribution of pathogens were compared.
    RESULTS A total of 223 pathogens were identified from the study objects, while 218 were identified by mNGS, including 46 bacterial strains, 120 M. pneumoniae strains, 51 viral strains, 1 fungal strain, and 125 strains were identified by the traditional method, including 7 bacterial strains, 27 viral strains, and 91 M. pneumoniae strains. The detection rates of bacteria, viruses, and M. pneumoniae by mNGS were significantly higher than those by traditional methods (P < 0.05).
    CONCLUSION Children with M. pneumoniae clinically tend to have mixed infections. Compared with traditional pathogen detection method, mNGS takes less time and has higher diagnostic efficiency.

     

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