Abstract: Under the background of increasingly severe drug-resistant bacteria infections, protracted course of chronic wounds has become clinical difficulties disturbing both patients and doctors. With the unique advantages of broad antibacterial spectrum, low drug resistance rate and high safety, polyhexanide antibacterial agent has gradually become a major approach for treatment and nursing of chronic wounds. The application of polyhexamethylene biguanide (PHMB) antimicrobial agents in treatment and nursing of chronic wounds was reviewed in the article, including the antibacterial properties, comparisons with other antimicrobial agents, actual clinical practice and potential side effects, aiming to guide the further application of PHMB antimicrobial agents in treatment of chronic wounds.