HPV16和HPV18亚型感染miR-196a表达水平及其与宫颈癌生存关系的生物信息学分析

Expression level of miR-196a in patients with HPV16 and HPV18 subtypes infections and bioinformatics analysis of its association with survival of cervical cancer

    摘要
  • 摘要:
    目的 探索高危型人乳头瘤病毒(HPV)16或18亚型感染后宫颈细胞中miR-196a的表达水平。
    方法 利用基因表达综合数据库(GEO)筛选HPV16或18亚型阳性宫颈癌细胞与正常宫颈细胞中表达差异miRNAs；利用在线生物学软件 https://kmplot.com/analysis/分析差异最大的miRNA表达情况与宫颈癌生存期的关系；收集经实时荧光定量聚合酶链式反应(qPCR)基因分型检测HPV16或18亚型阳性的宫颈拭子标本作为研究对象，以同期体检人群中经qPCR基因分型检测HPV16或18亚型阴性的宫颈拭子标本作为阴性对照，采用qRT-PCR方法检测宫颈细胞中的miR-196a水平，数据处理使用2-△△Ct法。
    结果 通过对GSE86100数据做差异分析发现miR-196a在HPV16或18亚型阳性宫颈细胞中表达降低(log2FC=-6.60，P < 0.001)，miR-3188在HPV16或18亚型阳性宫颈细胞中表达显著增加(log2FC=6.22，P < 0.001)；利用在线分析工具 https://kmplot.com/analysis发现miR-196a表达高的宫颈癌患者生存期较miR-196a表达低的宫颈癌患者生存期短(HR=1.87，95%CI：1.17~3.00，P=0.008)，而miR-3188与宫颈癌患者生存期无相关性(HR=1.47，95%CI：0.92~2.37，P=0.110)；特异性qRT-PCR对检测结果显示HPV16阳性、HPV18亚型阳性的宫颈细胞中miR-196a表达水平为(0.93±0.09，0.51±0.07)较正常对照组(1.89±0.13)降低(P < 0.05)，与测序分析结果一致。
    结论 HPV16或18亚型感染宫颈细胞后可导致miR-196a表达降低，且miR-196a表达水平与宫颈癌患者生存期呈负相关。

     

    Abstract:
    OBJECTIVE To explore the expression level of miR-196a in cervical cells infected with high-risk human papillomavirus (HPV) 16 and 18.
    METHODS The Gene Expression Omnibus (GEO) was used to screen for differentially expressed miRNAs between HPV 16 or 18-positive cervical cancer cells and normal cervical cells. Online biological software https://kmplot.com/analysis/ was utilized to analyze the relationship between the most differentially expressed miRNA and the overall survival of cervical cancer patients. Cervical swab samples positive for HPV 16 or HPV 18, detected by real-time fluorescent quantitative polymerase chain reaction (qPCR) genotyping, were collected as the study subjects. Cervical swab samples from the same period of physical examination population that were negative for HPV 16 or HPV 18 by qPCR genotyping served as negative controls. The qRT-PCR method was employed to detect the level of miR-196a in cervical cells, with data processed via the 2-△△Ct method.
    RESULTS Differential analysis of the GSE86100 data revealed that miR-196a expression decreased in HPV 16 or HPV 18-positive cervical cells (log2FC=-6.60, P < 0.001), while miR-3188 expression significantly increased (log2FC=6.22, P < 0.001). Using online analysis tools https://kmplot.com/analysis, it was found that cervical cancer patients with high miR-196a expression had a shorter overall survival compared to those with low miR-196a expression (HR=1.87, 95%CI: 1.17-3.00, P=0.008). However, there was no correlation between miR-3188 and the overall survival of cervical cancer patients (HR=1.47, 95%CI: 0.92-2.37, P=0.110). The results of specific qRT-PCR testing showed that the expression levels of miR-196a in cervical cells positive for HPV 16 and HPV 18 were 0.93±0.09 and 0.51±0.07, respectively, which were lower than those in the normal control group (1.89±0.13)(P < 0.05), consistent with the sequencing analysis results.
    CONCLUSIONS Infection of cervical cells with HPV 16 or HPV 18 can lead to decreased expression of miR-196a, and the expression level of miR-196a is negatively correlated with the overall survival of cervical cancer patients.

     

    • HTML全文
    • 参考文献(25)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回
下载中心