Abstract:
OBJECTIVE To analyze the microbial community structure in Bronchoalveolar Lavage Fluid (BALF) using high-throughput sequencing technology, with a particular focus on the abundance and distribution of Staphylococcus aureus.
METHODS A total of 1, 650 bronchoalveolar lavage fluid (BALF) samples were collected from the First People′s Hospital of Yunnan Province between 2022 and 2023. S. aureus gene detection was performed using BALF culture, isothermal amplification and microfluidic technology. Representative 21 S. aureus-positive samples were grouped into S. aureus pneumonia group, SARS-CoV-2 co-infection group and chronic obstructive pulmonary disease (COPD) group, followed by 16S rDNA amplicon high-throughput sequencing.
RESULTS The microbial community compositions among the three groups exhibited significant differences. Species-level hierarchical clustering analysis revealed that S. aureus was more frequently detected and accounted for a higher proportion in the S. aureus pneumonia group, while the COPD group was dominated by Achromobacter. In contrast, the SARS-CoV-2 co-infection group was mainly composed of Methylobacterium aquatique and Achromobacter. Only in the S. aureus pneumonia group was S. aureus the dominant bacterium.
CONCLUSION The composition of the BALF microbiota is closely related to different disease states, providing an important reference for a deeper understanding of the microbiological basis of pulmonary infectious diseases.
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