Abstract: In the multi-campus development and construction of public hospitals, hospital-associated infection management has consistently faced escalating difficulties and challenges. Throughout the long-term exploration and practice, the Second Affiliated Hospital Zhejiang University School of Medicine has adhered to the principles of "integrated planning and deployment, homogeneous and homologous training, information and data sharing, and a focus on professional features". By continuously addressing emerging infection control risk points and weak points, overcoming barriers posed by dispersed human resources, and balancing homogeneity with individualization, the hospital has actively promoted integration and collaborative development across its campuses. This approach has significantly contributed to the sustained improvement of healthcare service capacity and quality across multiple campuses. From the novel perspective of symbiosis theory, this paper analyzes the symbiotic dilemmas in multi-campus hospital-associated infection management, summarizes practical experiences, and explores future directions, aiming to provide references for other public hospitals in multi-campus hospital-associated infection management.