Abstract: The formation of bacterial biofilm imposes a formidable challenge to public health. Quorum sensing (QS), as a bacterial communication system with the capacity to sense bacterial density and modulate gene expression, can regulate the formation of bacterial biofilm by regulating the secretion of bacterial basic substances and extracellular proteins and affecting the bacterial motility. At present, some QS inhibitors (QSI) from natural sources and synthesized QSI have become potential choice for treatment of bacterial infections for their inhibiting the formation of bacterial biofilm through inhibition of QS signaling pathways. The article focuses on the progress of research on the effects of QS and QSI on the formation of bacterial biofilm.