呼吸道纹带棒状杆菌药物敏感性及全基因组测序

Drug susceptibility and whole-genome sequencing analysis of Corynebacterium striatum from respiratory tract

    摘要
  • 摘要:
    目的 分析呼吸道纹带棒状杆菌的分子流行病学特征和病原生物学特点, 揭示其耐药机制和毒力特征, 为临床防治提供科学依据。
    方法 收集2021年1月-2024年1月广东省中医院大学城医院分离的96株呼吸道纹带棒状杆菌, 采用基质辅助激光解吸电离飞行时间质谱鉴定, 肉汤稀释法检测11种抗菌药物的敏感性, 结晶紫染色法评估生物膜形成能力。选取22株进行全基因组测序, 构建核心基因组单核苷酸多态性(SNP)系统发育树, 分析耐药及毒力基因。
    结果 96株菌中, 多重耐药率83.33%(80/96)；青霉素、环丙沙星、头孢曲松、红霉素、克林霉素耐药率均>50%。生物膜形成能力呈异质性, 其中强、中、弱形成能力分别占7.29%(7株)、27.08%(26株)和65.63%(63株)。全基因组测序检测出11种耐药基因和5种毒力基因。22株中, 12株为已知ST型(ST23/ST14/ST33)；10株为未报道新基因型。
    结论 纹带棒状杆菌多重耐药率高, 基因型呈现区域聚集性, 新发现的基因型提示可能存在本地化进化。耐药基因谱与表型耐药率不完全匹配, 毒力基因与生物膜形成能力无显著关联, 需进一步研究其调控机制。应扩大监测范围以明确新基因型的临床意义及传播风险。

     

    Abstract:
    OBJECTIVE To analyze the molecular epidemiological and pathogenic biological characteristics of Corynebacterium striatum from the respiratory tract, reveal its drug resistance mechanisms and virulence features, and provide scientific evidence for clinical prevention and treatment.
    METHODS From Jan. 2021 to Jan. 2024, 96 strains of C. striatum from the respiratory tract were collected from the University City Hospital of Guangdong Provincial Hospital of Traditional Chinese Medicine. These strains were identified through matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The sensitivity to 11 antibacterial drugs was tested by the broth dilution method, and the biofilm formation ability was assessed by the crystal violet staining method. Twenty-two strains were selected for whole-genome sequencing and a core genome single nucleotide polymorphism (SNP) phylogenetic tree was constructed to analyze drug resistance and virulence genes.
    RESULTS Among the 96 strains, the multidrug-resistance rate was 83.33% (80/96). The resistance rates to penicillin, ciprofloxacin, ceftriaxone, erythromycin and clindamycin were all >50%. The biofilm formation ability was heterogeneous, with strong, moderate and weak formation abilities accounting for 7.29% (7 strains), 27.08% (26 strains) and 65.63% (63 strains), respectively. Whole-genome sequencing detected 11 drug resistance genes and 5 virulence genes. Among the 22 strains, 12 belonged to known ST types (ST23/ST14/ST33), and 10 were unreported new genotypes.
    CONCLUSIONS C. striatum has a high multidrug-resistance rate, and its genotypes show regional clustering. The newly discovered genotypes suggest possible localized evolution. The drug resistance gene profile does not completely match the phenotypic drug resistance rate, and there is no significant correlation between virulence genes and biofilm formation ability. Further research is needed to investigate their regulatory mechanisms. The monitoring scope should be expanded to clarify the clinical significance and transmission risk of the new genotypes.

     

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