OBJECTIVE To investigate the prevalence of multidrug-resistant bacteria infection/colonization in medical institutions of Pudong New Area and provide scientific evidence for formulating effective prevention and control measures.

METHODS Comprehensive monitoring of multidrug-resistant bacterial infections was conducted in 10 medical institutions of Pudong New Area, covering case samples, environmental samples and colonization carriage among healthcare workers.

RESULTS Multidrug-resistant bacteria were widely prevalent in medical institutions in Pudong New Area. Carbapenem-resistant Acinetobacter baumannii (CRAB) and carbapenem-resistant Klebsiella pneumoniae (CRKP) were the dominant strains, with detection rates of 35.49% and 28.41%, respectively. ICU cases accounted for over 40%, and the detection rate showed a significant increasing trend (P=0.007). The detection rate of A. baumannii in the environment was 5.24% (66/1 260), mainly contaminating contact surfaces such as hands and faucets. Colonization with drug-resistant bacteria was observed among healthcare workers, with 2 out of 3 co-colonized individuals carrying both Staphylococcus aureus and A. baumannii, indicating complex transmission routes. The resistance rate was highest in case samples, followed by environmental samples, and relatively lowest in colonization samples from healthcare workers. Clinical isolates exhibited extreme resistance to key drugs, with CRAB showing over 96.8% resistance to carbapenems and CRKP showing 100.00% resistance to ampicillin.

CONCLUSIONS The prevalence of multidrug-resistant bacteria in medical institutions of Pudong New Area is severe, particularly in intensive care units, which require special attention. Colonization among healthcare workers suggests potential risks for drug-resistant bacterial transmission, necessitating further strengthening of hand hygiene, environmental cleaning and disinfection, rational use of antibacterial agents and other comprehensive prevention and control measures.