Abstract: OBJECTIVE To define the genomic characteristic and prevalence trends of carbapenem-resistant Klebsiella pneumoniae(CRKP) isolated from a three-A hospital of Shandong Province. METHODS The clinical characteristics of the clinical Klebsiella pneumoniae isolates that were from Zaozhuang Municipal Hospital from Mar. 2023 to Feb. 2024 were retrospectively analyzed, and whole-genome sequencing(WGS) analysis was performed for 22 strains of CRKP that were randomly extracted from the strains isolated from non-sputum specimens. The molecular epidemiological characteristics of the CRKP strains were made completed through multilocus sequence typing(MLST) and analysis of capsular serotypes, drug resistance genes and virulence factors. RESULTSTotally 1 654 strains of K.pneumoniae were clinically isolated, 196 of which were CRKP. The patients who were detected with CRKP mainly came from intensive care unit and geriatrics department; the strains were mainly isolated from sputum and urine specimens. WGS analysis of the 22 strains of CRKP isolated from non-sputum specimens sources, 21 strains consisted of two clusters: ST11-KL25 and ST11-KL64, all of which carried bla_KPC-2 and iucA genes, 1 strain of ST11-KL25(ZY-66)carried peg344 and rmpA genes at the same time; 1 strain of ST25-KL2 carried bla_NDM-5 and did not carry the known virulence genes. CONCLUSION There is concurrent prevalence of ST11-KL25 and ST11-KL64 strains carrying bla_KPC-2 in the hospital. The ST11-KL25 ha certain transmission capacity, which differs some what from the domestic reports. There is a possiblity of cross-transmission involving ST11 strains, which the hospital infection control department and key clinical departments should attach great importance to.