Abstract: OBJECTIVE To clarify the incidence of neglected perforation (NP) of sterile gloves during laparoscopic radical resection of gastrointestinal cancer and analyze the influencing factors. METHODS A total of 1198 sterile surgical gloves that were used during laparoscopic radical resection of gastrointestinal cancer in Suqian Hospital of Jiangsu Provincial People's Hospital from Jan. 2023 to Dec. 2024 were enrolled in the study. The incidence of NP and the perforated sites of the gloves were statistically analyzed, and statistical analysis was performed for the risk factors for the NP of the gloves. RESULTS Of the 1198 gloves that were enrolled in the study, 116 were detected with NP, with the detection rate 9.68%. The detection rate of NP of the right-hand gloves was higher than that of the left-hand gloves(P＜0.05). The sites that were most likely to have NP were as follows: index finger, thumb and palm. The risk factors for NP of the gloves, including intraoperative pneumoperitoneum pressure of CO₂ no less than 14 mmHg, clear contamination with blood or body fluid, failed suction of intraabdominal smog or blood in a timely manner, body mass index of the patient no less than 28 kg/m² and operation duration no less than 180 min, were screened out by the univariate analysis of multivariate logistic analysis (P＜0.05); while the use of double-layer gloves was a protective factor for the prevention of NP(P＜0.05). CONCLUSIONS The incidence of NP is high among the gloves that are used during laparoscopic radical resection of gastrointestinal cancer. The high pneumoperitoneum pressure of CO₂ during surgery, clear contamination with blood or body fluid, failed suction of intraabdominal smog or blood, obesity of the patient and operation duration no less than 180 min are the risk factors for NP, while the use of double-layer gloves may reduce the incidence of NP.