Abstract:
OBJECTIVE To evaluate the sterilization effect of a self-developed dielectric barrier discharge plasma device on drug-resistant bacteria and common pathogenic microorganisms.
METHODS According to national and health industry standards, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Bacillus subtilis var. niger spores and methicillin-resistant S. aureus (MRSA) were selected as the research subjects. The sterilization effect of the plasma device was evaluated with qualitative plate culture method and carrier quantitative sterilization method, and the ultrastructural changes of bacteria after plasma treatment were observed with scanning electron microscope. A mouse model of drug-resistant bacterial infected wound was established, and the sterilization effect of plasma treatment on bacteria in infected wounds in vivo was detected on the 3rd and 7th days after infection.
RESULTS After two minutes of treatment with the plasma device, the average killing log values for S. aureus, E. coli, P. aeruginosa and MRSA were >3.00. Scanning electron microscope revealed ruptured bacterial cell membranes and leakage of contents after plasma treatment. In the mouse model of drug-resistant bacterial infected wound, alternate-day plasma treatment significantly reduced the bacterial load in the wound on the 3rd and 7th days.
CONCLUSION The self-developed dielectric barrier discharge plasma device can effectively kill common pathogenic microorganisms and MRSA, providing ideas for the treatment of clinical wound infections, especially those caused by drug-resistant bacteria.
下载: