OBJECTIVE  To survey the current status of standardized management of hospital-associated infections in operating rooms of traditional Chinese medicine （TCM） hospitals in Guangdong Province, providing references for further standardizing the management of hospital-associated infections in operating rooms.

METHODS  A questionnaire survey and on-site verification were conducted from May 2021 to Jan. 2022 to assess the standardized management of operating rooms in 58 secondary and tertiary TCM （integrated Chinese and Western medicine） hospitals in Guangdong Province.

RESULTS  The survey covered 32 tertiary hospitals and 26 secondary hospitals. Clean operating rooms were available in 46 hospitals, with a higher proportion in tertiary hospitals （93.75%） than in secondary hospitals （P＜0.05）. Fifty-six hospitals had established a three-tier hospital infection management organization. Forty-nine hospitals had comprehensive hospital-associated infection prevention and control guidelines for operating rooms, with tertiary hospitals outperforming secondary hospitals （P＜0.05）. Most hospitals complied with standards in training, aseptic techniques, hand hygiene, sterile item management, surgical instrument and fabric dressing management, environmental surface cleaning and disinfection and medical waste storage （93%−100%）. However, low compliance rates were observed in personnel and equipment management, clean operating room operation and maintenance and medical waste disposal, particularly in intraoperative personnel movement （34.48%）, preoperative patient bathing （68.97%）, disinfection of fiberoptic bronchoscopes, laryngoscopes and laryngeal masks （82.75%）, cleaning and disinfection of air conditioning system （67.39%） and fresh air system vent/return vent （76.09%）, primary filter replacement （80.43%） and dedicated medical waste transport routes （84.48%）.

CONCLUSIONS  The hospital-associated infection organization management system is relatively well-established, with tertiary hospitals having a higher rate of clean operating rooms. Secondary hospitals need improvement in the hospital-associated infection prevention and control guidelines for operating rooms. Common issues across all hospital levels include inadequate intraoperative personnel movement management, preoperative patient skin preparation and clean operating room operation and maintenance, necessitating targeted management and supervision to ensure medical quality and patient safety.