Abstract: OBJECTIVE To investigate the characteristics of human papillomavirus (HPV), Ureaplasma urealyticum (UU), Chlamydia trachomatis (CT), and Neisseria gonorrhoeae (NG) infections in Sanya so as to provide scientific bases for diagnosis, treatment, prevention and control of infectious diseases. METHODS The patients who were treated and tested for the first time in the hospital from Jan. 2022 to Dec. 2024 were enrolled in the study, the incidence rates of HPV, UU, CT and NG infections, age, modes of mixed infections and associations among the infections with different pathogens were retrospectively analyzed. RESULTS The positive rates of HPV, UU, CT and NG were 22.74%, 36.34%, 9.54% and 6.55%,respectively; there was significant difference in the positive rate among the four types of pathogens(P＜0.001). The gender analysis showed that the positive rate of Uu was far higher among the female (46.64%) than among the male (27.61%) (P＜0.001); however, the positive rates of GT and NG of the male were respectively 12.93% and 10.12%, higher than 5.57% and 1.72% of the female( all P＜0.001). The age distribution indicated that the positive rate of Uu was highest (more than 37.00%) among the patients 15 to 50 years of age; the patients more than 60 years of age ranked the first place (32.10%) for the positive rate of HPV; the incidence rates of CT and NG infection of the patients 15 to 30 years of age were respectively 13.70% and 9.49%, higher than those of other age groups(P＜0.001). As for the condition of mixed infections, the incidence of single HPV genotype infection was dominant (17.17%); the incidence of UU+CT infections was highest (4.47%) among the mixed modes of UU, CT and NG. Furthermore, the positive rate of UU was higher among the patients with HPV infection than among the patients without HPV infection(χ² = 7.620,P=0.006). CONCLUSIONS The study lays a solid laboratory evidence for the prevalence of HPV, UU, CT and NG infections and has significant value in developing public health strategies and taking clinical intervention measures.