Abstract: OBJECTIVE To explore the effect of limited fluid resuscitation (LFR) combined with early sedation and analgesia on dynamic changes of myocardial injury markers of the patients with septic shock (SS). METHODS A total of 150 patients with SS who were treated in Changshu Hospital of Traditional Chinese Medicine from Jan. 2022 to Dec. 2024 were recruited as the research subjects and were randomly divided into the study group (LFR combined with early sedation and analgesia) and the control group (adequate fluid resuscitation combined with early sedation and analgesia), with 75 cases in each group. The Acute Physiology and Chronic Health Evaluation II (APACHE II) score, sequential organ failure assessment (SOFA), hemodynamics indicatorsplasma viscosity (PV), hematocrit (HCT), and fibrinogen (FIB), inflammatory factorsinterleukin-6 (IL-6), IL-1, tumor necrosis factor-α (TNF-α), and C-reactive protein (CRP), and myocardial injury indicatorscardiac troponin I (cTnI), creatine kinase isoenzyme (CK-MB), brain natriuretic peptide (BNP)were observed and compared between the two groups before the treatment and after the treatment for 24, 48, and 72 hours. RESULTS The total effective rate of the study group was 89.33%, higher than 76.00% of the control group(P＜0.05). There were significant differences in APACHE II score, SOFA score, PV, HCT, FIB, IL-6, IL-1, TNF-α, CRP, cTnI, CK-MB and BNP between the two groups at the time points (all P＜0.05). The APACHE II score, SOFA score, PV, HCT, FIB, IL-6, IL-1, TNF-α, CRP, cTnI, CK-MB and BNP were lower in the study group than in the control group after the treatment (all P＜0.05). CONCLUSIONS LFR combined with early sedation and analgesia can remarkably improve the clinical therapeutic effect and hemodynamic reactions of the patients with SS, reduce the scores of APACHE II and SOFA, and decrease the levels of myocardial injury indictors and inflammatory factors.