Abstract: OBJECTIVE To analyze the changing trends of disease of burden due to multidrug-resistant organisms (MDROs) infections in China from 1990 to 2021 so as to provide bases for development of prevention and control policies. METHODS The data regarding to the burden of disease associated with MDROs infections in China (except Taiwan) from 1990 to 2010 were collected from the GBD database; the number of deaths, mortality rate, DALYs and the rate of DALYs were taken as the indexes for evaluation of the burden of diseases. The changing trends were observed and compared among the different MDROs infections and the different age groups. RESULTS The proportion of death cases related to MDROs infections showed an upward trend in China from 1990 to 2021 (P<0.001). The disease burdens of methicillin-resistant Staphylococcus aureus (MRSA), carbapenem-resistant Acinetobacter baumannii (CRAB) and carbapenem-resistant Pseudomonas aeruginosa (CRPA) ranked the top 3; MRSA rose rapidly after 1990 and showed a slight decline after 2016, CRAB declined slightly after 2010, and CRPA remained relatively stable after 2010; carbapenem-resistant Escherichia coli (CRECO) began to increase slowly after 2010, while vancomycin-resistant Enterococcus (VRE) remained consistently low; carbapenem-resistant Klebsiella pneumoniae (CRKP) increased markedly after 2010; regarding the disease burden of MDROs infections across the age groups, neonates and children aged less than 5 years old showed a downward trend, whereas adults aged no less than 70 years old showed an upward trend. CONCLUSION In the burden of disease of MDROs infection in China from 1990 to 2021, MRSA, CRAB and CRPA have been in the top three, but they are basically in the trend of decreasing or very small increase after 2010, VRE has been at a low level, CRECO is slowly increasing, and CRKP is rapidly increasing, which needs to be further paid attention to and strengthened in the prevention and control of CRKP infections among the elderly population.