Abstract: OBJECTIVE To investigate the detection rate, serotype distribution and antimicrobial resistance trends of Salmonella in fecal specimens from children at Hunan Children's Hospital, and to provide reference for the diagnosis and treatment of Salmonella infections. METHODS A retrospective analysis was conducted on the detection rate, serotype distribution and antimicrobial resistance trends of Salmonella in children's fecal specimens from 2022 to 2024. Chi-square tests were employed for data analysis and comparison. RESULTS The number of Salmonella isolates from 2022-2024 was 121, 139 and 123, respectively. Salmonella typhimurium had the highest detection rate at 67.62% (259/383). The detection rate of Salmonella peaked from May to Oct., accounting for 78.85% (302/383). The male-to-female detection ratio was 1.62∶1 (237/146). The detection rates varied across age groups, with the highest rate (61.62%, 236/383) observed in children aged 28 days to 1 year. Over the three years, antimicrobial resistance among the 383 Salmonella isolates showed no significant changes, with resistance rates of 42.04% to sulfamethoxazole/trimethoprim and 40.37% to ceftriaxone. CONCLUSIONS In this region, male children under 1 year old are more susceptible to Salmonella infections during summer and autumn, with S. typhimurium being the predominant serotype. Carbapenem-resistant strains first emerged in 2023. Based on antimicrobial susceptibility testing, third-generation cephalosporins may be prioritized for treatment. Clinicians should monitor local Salmonella infection characteristics and resistance trends to ensure early diagnosis and rational antimicrobial use.