Abstract: OBJECTIVE To investigate the epidemiological characteristics and drug resistance of carbapenem-resistant Klebsiella pneumoniae (CRKP) in 9 medical institutions of Pudong New Area of Shanghai. METHODS The clinical data and some patients' and environmental samples (object surface, hands and nasal cavities of healthcare workers) were collected from the patients with CRKP infection who were treated in key departments of the 9 medical institutions of Pudong New Area of Shanghai from Jun. 2024 to May 2025. The epidemiological characteristics and drug resistance rates were observed. RESULTS A total of 508 patients with CRKP infection were enrolled in the study, the clinical data were collected from the patients, the mean age was (70.38±17.29) years old; 36.41% of the strains were isolated from ICU, and 45.67% were isolated from sputum specimens; there was significant difference in the distribution of infections among the departments and specimen types (P＜ 0.05). A total of 23 (1.33%) of 1728 environmental object surface samples were tested positive for CRKP, predominantly found on medical devices, cleaning supplies, shared items, and patient-contact objects. Additionally, 17 of 864 samples from the healthcare workers' hands and nasal cavities were tested positive for CRKP with the total isolation rate 1.97%, 3 of which were from hands, and 14 were from nasal cavities. ST11 (54.17%) was the predominant serotype among the strains isolated from the patients, and ST15 (7.50%) was dominant among the strains isolated from environment; ST15 was concurrently identified in both clinical cases and environmental samples. The drug resistance rats of the CRKP strains to most of the antibiotics were high, the drug resistance rate to ampicillin was highest; there were significant differences in the drug resistance rates to 13 types of antibiotics between the environmental strains and the strains from the patients (P＜0.001). CONCLUSIONS The ICU patients and elderly patients are dominant among the patients with CRKP infection in Pudong New Area. ST11 is the predominant clone type, and the strains exhibit high multi-drug resistance rates. There is significant difference in the drug resistance spectrum between the environmental strains and the strains from the patients, which indicates that the current environmental disinfection and infection control measures are effective. It is necessary to strengthen the active surveillance of key departments and intensify the environmental disinfection and hand hygiene so as to prevent the spread of CRKP strains.