Abstract: OBJECTIVE To investigate the antimicrobial resistance and genotypes of carbapenem-resistant Klebsiella pneumoniae (CRKP) clinically isolated from children. METHODS A total of 29 CRKPs were isolated from hospitalized children in Xuzhou Medical University Affiliated Hospital and Xuzhou Children's Hospital from Jun. 2015 to Feb. 2018. The bacteria were identified using Bruker's bacterial mass spectrometer. The sensitivity analyzer VITEK2 Compact was used to detect the minimum inhibitory concentration (MIC) of the strains. The common carbapenem resistance genes (bla_KPC, bla_SME, bla_GES, bla_VIM,bla_IMP, bla_NDM,bla_OXA-48), extended-spectrum β-lactamase resistance genes (bla_SHV,bla_TEM,bla_CTX-M-1group,bla_CTX-M-2group, bla_CTX-M-8group, bla_CTX-M-9group), and cephalosporin resistance genes (bla_MOX, bla_CIT, bla_DHA, bla_ACC, bla_EBC, bla_FOX) were amplified by PCR. RESULTS The resistance rates of 29 strains of CRKP to meropenem and imipenem were all 100%. The resistance rates to amikacin, minocycline, levofloxacin and aztreonam were respectively 3.45%, 10.34%, 17.24%, and 93.10%. All strains were resistant to cephalosporins and enzyme inhibitors, and sensitive to both polymyxin and tigecycline. All 29 strains of CRKP carried carbapenem-resistant genes, and the positive rate of bla_NDM-5 gene was the highest, accounting for 51.72% (15/29), the positive rate of bla_NDM-1 gene was 31.03% (9/29), and the positive rate of bla_{KPC- 2} gene was 17.24% (5/29). One strain carried both bla_KPC-2 and bla_IMP-4. CONCLUSION The CRKP strains isolated from children in this region are multi-drug resistant bacteria. The mechanism of carbapenems resistance in clinically isolated Klebsiella pneumoniae is mainly the production of NDM-5 carbapenemase.