Abstract: OBJECTIVE To observe the relationship between adverse pregnancy outcomes during pregnancy and the infection of Toxoplasma gondii, rubella virus, cytomegalovirus and herpes simplex virus (TORCH) in early pregnancy and late pregnancy, so as to provide the basis for clinical treatment. METHODS A total of 150 cases of pregnant women with adverse pregnancy outcomes were selected as observation group and 150 cases of pregnant women without adverse pregnancy outcomes were selected as control group in hospital from Jan. 2014 to Dec. 2015. Early pregnancy maternal were 100 cases and late pregnancy maternal were 50 cases in the two groups. Enzyme-linked immunosorbent assay was used to determine the serum IgG and IgM antibodies of T. gondii, rubella virus, cytomegalovirus and herpes simplex virus. RESULTS The IgG positive rates of T. gondii, rubella virus, cytomegalovirus and herpes simplex virus in observation group were higher than those in control group in early pregnancy and late pregnancy, and the IgM positive rates of T. gondii, rubella virus, cytomegalovirus and herpes simplex virus in observation group were higher than those in control group in early pregnancy and late pregnancy (P<0.05). The positive rates of IgG+IgM of T. gondii, rubella virus, cytomegalovirus and herpes simplex virus in observation group were higher than those in control group in early pregnancy and late pregnancy (P<0.05). CONCLUSION Pregnancy adverse pregnancy outcomes are closely related to TORCH infection in early pregnancy and late pregnancy. TORCH screening in pre-pregnancy and pregnancy is important to reduce adverse pregnancy outcomes.