Abstract: OBJECTIVE To explore the effect of Xintong Granules on inflammatory response and protection of myocardial damage of the mice with viral myocarditis induced by Coxsackie virus.METHODS Totally 150 healthy male BALB/c mice were randomly divided into the control group, the myocarditis group, the ribavirin group, the high-dose Xintong granule group and the low-dose Xintong granule group, with 30 mice in each group. The ribavirin group, the high-dose Xintong granule group and the low-dose Xintong granule group were respectively treated with gavage, the other two groups were given normal saline. All of the groups were injected with Coxsackie Group B Type 3（ CVB3） virus to establish viral myocarditis mouse models on Day 7 except for the control group, the groups were respectively treated with same drugs after 7-12 days. The levels of cardiac troponin I（cTnI）, creatine kinase isoenzyme MB（CK-MB）, creatine kinase（CK）, tumor necrosis factor-α（TNF-α） and interferon-γ（IFN-γ） as well as the expression levels of malondialdehyde（MDA）, superoxide dismutase（SOD）, glutathione peroxidase（GSH-Px） and CVB3 mRNA in myocardial tissues were detected by using enzyme-linked immunosorbent assay.RESULTS The levels of cTnT, CK, CK-MB, MDA, TNF-α, IFN-γ and CVB3 mRNA of the myocarditis group were significantly higher than those of the control group（P＜0.05）; the levels of SOD, GSH-Px, IL-4 and IL-10 of the myocarditis group were significantly lower than those of the control group（P＜0.05）. The levels of cTnT, CK, CK-MB, MDA, TNF-α, IFN-γ and CVB3 mRNA of the high-dose Xintong granule group and the low-dose Xintong granule group were significantly lower than those of the myocarditis group（P＜0.05）; the levels of SOD, GSH-Px, IL-4 and IL-10 of the high-dose Xintong granule group and the low-dose Xintong granule group were significantly higher than those of the myocarditis group, and the levels of above indexes of the high-dose Xintong granule group changed more remarkably（P＜0.05）.CONCLUSION The myocarditis models of mice are successfully established by injecting CVB3 virus. The myocardial biochemical indexes and inflammatory response of the mice are significantly reduced through the intervention of Xintong Granules（especially the high-dose）, which may be associated with the inhibition of inflammatory response.