HPV16 E6及E7蛋白在宫颈癌组织中的表达与病理特征的相关性研究

Correlation between expression of HPV16 E6 and E7 protein in cervical carcinoma tissues and pathological characteristics

    摘要
  • 摘要: 目的 探讨人乳头瘤病毒(HPV)16 E6及E7蛋白在宫颈癌前病变中宫颈癌组织的表达与病理特征的相关性,分析引起宫颈癌病变中的作用机制。方法 收集2014年1月-2015年10月在医院手术的宫颈癌及宫颈上皮内瘤变(CIN)患者各60例,采用免疫组化Envision二步法对病变标本组织中HPV16 E6、E7蛋白表达进行检测。结果 宫颈癌组织中E6、E7表达阳性率分别为90.00%、88.33%,明显高于CIN组的68.33%、71.67%,差异有统计学意义(P＜0.05); 鳞癌、分期Ⅲ-Ⅳ期患者宫颈癌组织中E6、E7表达阳性率分别为97.62%、97.62%、100.00%、83.87%,明显高于腺癌、分期Ⅰ-Ⅱ期的72.22%、66.67%、79.31%、58.62%,差异有统计学意义(P＜0.05),E6、E7表达阳性率与癌细胞分化程度无关; Ⅲ期患者CIN组织中E6、E7表达阳性率分别为86.96%、86.96%,明显高于Ⅰ-Ⅱ级的56.76%、62.16%,差异有统计学意义(P＜0.05)。结论 HPV16 E6及E7蛋白在宫颈癌组织中阳性表达率较宫颈癌前病变明显增高,并与病理特征关系密切,检测HPV16 E6及E7蛋白有可能作为宫颈癌前病变与宫颈癌转归的指标。

     

    Abstract: OBJECTIVE To explore the relationship between the expression of human papilloma virus (HPV)16 E6 and E7 protein in cervical carcinoma tissues of patients with cervical precancerous lesions and observe the pathogenesis of cervical carcinoma.METHODS Totally 60 patients with cervical cancer and 60 patients with cervical intraepithelial neoplasia (CIN) who were treated in the hospital from Jan 2014 to Oct 2015 were enrolled in the study, then the HPV16 E6, E7 proteins in the lesion tissue species were detected by using immunohistochemistry Envision two-step method.RESULTS The positive rate of expression of E6 of the cervical cancer group was 90.00%, significantly higher than 68.33% of the CIN group; the positive rate of expression of E7 of the cervical cancer group was 88.33%, significantly higher than 71.67% of the CIN group(P＜0.05).The positive rate of expression of E6 of the patients with squamous carcinoma was 97.62%, significantly higher than 72.22% of the patients with adenocarcinoma; the positive rate of expression of E7 of the patients with squamous carcinoma was 97.62%, significantly higher than 66.67% of the patients with adenocarcinoma; the positive rate of expression of E6 of the stage Ⅲ-Ⅳ patients was 100.00%, significantly higher than 79.31% of the stage Ⅰ-Ⅱ patients; the positive rate of expression of E7 of the stage Ⅲ-Ⅳ patients was 83.87%, significantly higher than 58.62% of the stage Ⅰ-Ⅱ patients(P＜0.05).The positive rate of expression of E6, E7 was not associated with degree of cell differentiation.The positive rate of expression of E6 of the stage Ⅲ patients was 86.96%, significantly higher than 56.76% of the stage Ⅰ-Ⅱ patients; the positive rate of E7 of the stage Ⅲ patients was 86.96%, significantly higher than 62.16% of the stage Ⅰ-Ⅱ patients(P＜0.05).CONCLUSION The positive rates of expression of HPV16 E6 and E7 in the cervical carcinoma tissues are remarkably increased in the patients with cervical precancerous lesions and are closely associated with the pathological characteristics.The HPV16 E6 and E7 could be used as indicators for outcomes of cervical precancerous lesions and cervical cancer.

     

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