Abstract: OBJECTIVE In response to the unexpected outbreak of Corona Virus Disease 2019（COVID-19）, a new type of rapid movable isolation ward was developed. In this study, the performance of the isolation ward was tested in site inspection and data were analyzed. METHODS According to GB 50333 standard, the air filtration efficiency, ventilation frequency and static pressure difference of isolation room were detected. RESULTS According to the instruction of IFU, one person can complete the construction of isolation ward in 5 minutes, with the internal area of 6 m~2 or so. The plastic enclosure is used in the ward; both sides are completely closed, and there are air inlet cracks at the head and foot of the patient’s bed. After HEPA was activated, 0.5 μm and 5.0 μm particles in isolation ward showed a downward trend. The number of 0.5 μm particles decreased by 30.39% compared with before the opening of HEPA（P＜0.001）. The decrease of 5.0 μm particles was more obvious, 67.50% less than before HEPA opening（P=0.001）. The air intake volume of isolation ward was 200 m~3/h, and the ventilation rate was 15 times/h. There was a slight negative pressure（-0.5 Pa） in the isolation ward compared with that outside. When HEPA was running, the noise was 54.5 dB. In order to avoid the surface contaminated by hand touching, the non-hand touching automatic door system was used for entry and exit. CONCLUSION This rapid movable isolation ward has good air filtering efficiency. It can be applied for the emergency isolation of patients with respiratory tract infectious diseases or drug-resistant bacteria infection in large wards of medical institutions, so as to implement effective bedside isolation measures.