Abstract: OBJECTIVE To analyze the effect of optimized nursing on postoperative urinary tract infection in radical prostatectomy patients. METHODS A total of 42 patients with prostate cancer diagnosed in the First Affiliated Hospital of Nanjing Medical University from Jan 1, 2022 to Jul 1, 2022 were randomly divided into two groups: the control group and the experimental group, with 21 patients in each group. A conventional treatment plan was administered in the control group and an optimized treatment plan was administered in the experimental group. General data of the patients, the degree of urinary tract infection symptoms, duration of treatment, complications and patients' satisfaction scores were observed and recorded to comprehensively evaluate the effectiveness of optimized treatment. RESULTS After 7 days of treatment, the levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), procalcitonin (PCT) and C-reactive protein (CRP) in the experimental group were higher than those in the control group (P＜0.05). The expression levels of miRNA-15a, miRNA-34b and miRNA-103 in the experimental group were higher than those in the control group at 7 days after treatment (P＜0.05). The duration of infection treatment in the experimental group was shorter than that in the control group (P＜0.05). The satisfaction score of urinary tract infection treatment in the experimental group was higher than that in the control group (P＜0.05). CONCLUSION Optimized treatment for patients has a good therapeutic effect, shortens the treatment time, and improves the satisfaction with medical personnel in patients.