Abstract: OBJECTIVE To analyze the detection results, drug resistance and CYP1A2 gene polymorphism of pathogens causing urinary tract infection in patients with benign prostatic hyperplasia after operation. METHODS From Jan 2019 to May 2022, 206 cases of postoperative patients with benign prostatic hyperplasia in Shandong Taishan Hospital were enrolled as the research subject, and they were divided into the infected group (63 cases) and the uninfected group (143 cases) according to whether the urinary tract infection occurred in patients after operation.The general data, clinical features and CYP1A2 gene polymorphism between the two groups were compared, and the distribution and drug resistance of pathogenic bacteria in patients with urinary tract infection after prostatectomy were analyzed. RESULTS The proportion of diabetes mellitus patients combined with infection was higher than that of the uninfected group, and the hospital stay was longer than that in the uninfected group (P＜0.05).The percentage of allele A and genotype AA in the infected group was higher than that in the uninfected group (P＜0.05).Seventy-two pathogenic bacteria were isolated from 63 patients with urinary tract infection after prostatectomy, of which 41 were gram-negative bacteria, accounting for 56.94%.The highest detection rate was Escherichia coli, accounting for 27.78%.Thirty-one strains of gram-positive bacteria were detected, accounting for 43.06%, and Enterococcus faecium accounted for 18.06%.E.coli had high resistance to ampicillin, and.17 drug-resistant strains were detected.They were sensitive to cefoxitin, amicacin, piperacillin/tazobactam, cefepime, furanotoin, cefoperazone and imipenem.Enterococcus faecium was highly drug-resistant to penicillin G, and 11 drug-resistant strains were detected.It was sensitive to furantoin and vancomycin. CONCLUSION Postoperative urinary tract infection patients with prostatic hyperplasia were characterized by a high proportion of patients with diabetes and a long hospital stay.The main pathogenic bacteria are E.coli and Enterococcus faecium.The highly sensitive antibiotics administered is helpful to control the progression of the disease in patients as soon as possible.