Abstract: Influenza remains one of the most serious threats to human health recently. Accurate and early diagnosis of influenza virus infection are essential for rapid antiviral treatment so as to reduce flu-related morbidity and mortality during seasonal epidemics.At present, the common methods for detection of influenza virus include virus culture, antigen detection, nucleic acid detection, serology detection and second generation sequencing technology.The manuscript focus on the application of each method in detection of influenza virus.