Abstract: OBJECTIVE To explore the types of human papillomavirus (HPV) infection and observe the influence of multiple infections on cervical lesions. METHODS A total of 2 126 patients who received Thinprep cytologic test combined with HPV-DNA test in the hospital from Feb 2016 to Feb 2018 were recruited as the study objects. RESULTS Of the 2 126 subjects, 362 were positive for HPV, with the positive rate 17.03%; totally 17 subtypes were detected. The top 3 high-risk subtypes were HPV16(2.92%), HPV52(1.93%) and HPV56(1.88%), respectively. Among the low-risk subtypes of HPV, the incidence rate of HPV6 infection was the highest (0.75%). Of the 362 patients positive for HPV, 322 (15.15%) had single HPV infection, and 40 (1.88%) had multiple infections. The patients with NILM accounted for 67.13%, the patients with ASCUS 12.15%, the patients with LSIL 8.84%, the patients with HSIL 2.76%, the patients with reactive cell change complicated with inflammation 9.12%. The patients with chronic cervicitis 65.19%, the patients with low-grade epithelial lesions 18.23%, the patients with high-grade epithelial lesions 10.50%, the patients with infiltrating carcinomas 6.08%. The patients with different cervical pathological grades differed in the incidence of HPV infection. After the age was adjusted, the logistic regression analysis of the types of HPV showed that the infiltrating carcinomas was highly associated with the HPV 16 and HPV18 infection (P<0.05). CONCLUSION HPV16 and HPV18 may accelerate the progression of cervical lesions, the HPV infection is remarkably associated with the cervical lesions, and the cervical cancer HPV infection tends to be single HPV infection.