Abstract: OBJECTIVE To investigatet the effect of neonatal cytomegalovirus infection on liver function indicators, including total bilirubin（TBIL）, alanine aminotranspeptidase（ALT）, aspartate aminotranspeptidase（AST）, glutamyl transpeptidase（GGT） levels, serum immunoglobulin（Ig） IgG, IgA, IgM, and IgE levels and peripheral blood T lymphocyte subsets, and immune function. METHODS A total of 113 neonates with CMV infection admitted to the Women and Children Hospital of Hainan Province from Mar. 2016 to Jul. 2018 were selected as the study subjects, of which 75 children with latent CMV infection were assigned to latent groupand 38 children with active CMV infection were assigned to active group, and 50 healthy neonates were selected as control group. The changes of TBIL, ALT, AST, GGT, serum immunoglobulin, complement and T lymphocyte subsets（CD₄⁺, CD₈⁺, and CD₄⁺/CD₈⁺） in peripheral blood were detected in the three groups. RESULTS The liver function indexes including TBIL, ALT, AST and GGT in actvie group were（23.81±5.62）μmol/L,（58.37±17.23）U/L,（79.68±21.87）U/L, and（22.14±6.38）U/L, respectively, significantly higher than those in latency group of（9.12±2.26）μmol/L,（22.05±6.10） U/L,（46.17±12.56）U/L, and（17.95±4.11 U/L）, respectively and in control group（P＜0.05）, and the liver function of the latent group was significantly higher than that of the control group（P＜0.05）. There were no significant differences of serum immunoglobulin IgG, IgA, IgM and IgE levels among the three groups. The CD₄⁺ and CD₄⁺/CD₈⁺ levels in peripheral blood in the active group were（37.84±10.63） and（1.20±0.28）, respectively, lower than those in latent group（45.27±7.22） and（1.64±0.40）, respectively) and control group（P＜0.05）, while CD₈⁺ level in active group was significantly higher than that in latent group and control group（P＜0.05）. The levels of CD₄⁺ and CD₄⁺/CD₈⁺ in latent group were significantly lower than those in control group（P＜0.05）, and the CD₈⁺ level in tlatent group was（27.14±1.95）, significantly higher than that in control group（P＜0.05）. There was no significant difference of NK cells among the two groups（P=0.462）. CONCLUSION Cytomegalovirus infection can cause neonatal liver damage and inhibit cellular immune function, but has no significant effect on the body’s humoral immune function, of which active cytomegalovirus infection has the greatest impact on the body.