Abstract: Coagulase-negative Staphylococci (CoNS) are a group of gram-positive cocci incapable of producing coagulase, which distinguishes them from Staphylococcus aureus. CoNS are widely distributed in natural environment and are commonly found on skin, mucous membranes and other host-associated tissues. In recent years, CoNS have become one of the significant pathogenic factors for hospital-associated infections and have been particularly prevalent among the patients with impaired immune function. Although the pathogenicity of CoNS is generally considered lower than that of coagulase-positive Staphylococci, the widespread use of implanted medical devices and extensive use of antibiotics has led to increasingly serious problems of drug resistance, which has attached great importance to the hospital. The databases such as PubMed, Embase and CNKI were retrieved to reveal the drug resistance mechanisms of CoNS, aiming to push forward the research and development of new drugs against drug-resistant bacteria and emerging diagnosis and treatment measures and provide scientific bases for solving the problems of antibiotics resistance around the globe.