Abstract: OBJECTIVE To investigate the significance of serum interleukin-6（IL-6）, procalcitonin（PCT） and hypersensitive C-reactive protein（hs-CRP） in the diagnosis of neonatal bacterial pneumonia. METHODS 150 cases of diagnosed neonatal bacterial pneumonia in our hospital from Apr. 2015 to Feb. 2018 were selected and divided into bacterial infection group（78 cases） and non-bacterial infection group（72 cases）, respectively, according to the bacterial infection. Another 75 healthy newborns born at the same time were chosen as a control group. Serum IL-6, PCT and hs-CRP levels of patients at admission were compared among three groups. Receiver operating characteristic curve（ROC curve） were used to analyze the diagnositic value serum IL-6, PCT andhs-CRP in neonatal bacterial pneumonia. RESULTS At admission, the levels of IL-6, PCT and hs-CRP of children with bacterial infection were（253.46±53.25） pg/ml,（2.23±1.21） ng/ml and（23.46±1.21） ng/ml,respectively, significantly higher than those of non-bacterial infection group（19.42±10.24） pg/ml,（0.81±0.76） ng/ml, and（9.75±2.35） mg/L, respectively. and diagnosis of neonatal bacteria The sensitivity and specificity of hs-0.05-hs-CRP in diagnosing neonatal bacteria pneumonia were 79.49% and 84.62%, significantly higher than that of IL-6（P＜0.05）. The sensitivity and specificity of serum PCT in diagnosis of neonatal bacterial pneumonia were 83.33% and 88.46%, respectively, higher than hs-CRP（P＜0.05）. Whereas the sensitivity and specificity of PCT and IL-6 combined with hs-CRP in diagnosis of neonatal bacterial pneumonia were 92.31% and 93.59%, respectively, significantly higher than those of IL-6, PCT and hs-CRP（P＜0.05）. The area under ROC curve（AUC） of IL-6 was 0.821（95% CI: 0.683～0.864）, the AUC of PCT was 0.857（95% CI: 0.742～0.908）, the AUC of hs-CRP was 0.838（95% CI: 0.704～0.885）, respectively, while the AUC of IL-6 combined with PCT and hs-CRP was 0.893（95% CI: 0.783～0.952）,significantly better than that of the single detection（P＜0.05）. CONCLUSION The levels of serum IL-6, PCT and hs-CRP in patients with neonatal bacterial pneumonia are significantly elevated, and exhibited good efficacy in the diagnosis of neonatal bacterial pneumonia, however, the combined value of hs-CRP, PCT and IL-6 is more valuable.