Abstract: OBJECTIVE To investigate the current status of microbial contamination of ultrasound probe and transvaginal ultrasound probe. METHODS The samples were taken from the ultrasound probes and transvaginal probes that were cleaned and disinfected in ultrasound department of 5 hospitals in Beijing, the samples were submitted to the clinical laboratory within 4 hours for viable counting and identification of microbial species, meanwhile, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans were set as the target bacteria to calculate isolation rate. RESULTS Totally 173 samples were collected from the ultrasound probes in the 5 three-A hospitals in Beijing, 90.17% of ultrasound probes exceeded the total bacterial count standard. S.aureus was detected out in 8 samples, P.aeruginosa was detected out in 1 sample, and C.albicans was not detected. 98.41% of transvaginal ultrasound probes exceeded the total bacterial count standard, however, S.aureus, P.aeruginosa and C.albicans were not detected. CONCLUSION The condition of microbial contamination of the ultrasound probes and transvaginal probes is severe, the poor health condition may lead to the risk of nosocomial infection, and it is urgent to seek for appropriate disinfection method.