Abstract: OBJECTIVE To investigate the implementation of 1-hour bundle treatment strategy (1 h bundle) for the management of sepsis by medical staff in the intensive care unit (ICU), and to analyze the factors affecting the 28-day prognosis of patients with sepsis/septic shock. METHODS Patients who met the definition and diagnostic criteria of sepsis 3.0 in the intensive care unit (ICU), emergency intensive care unit (EICU), and respiratory intensive care unit (RICU) of the First Affiliated Hospital of Xinjiang Medical University from Jun. 2019 to Jul. 2022 were selected and divided into 100 cases in the survival group and 91 cases in the death group according to the 28-day prognosis. The distribution characteristics and infection sites of pathogens, 1 h bundle execution, and 28-day prognosis were documented. Risk factors affecting prognosis were analyzed and receiver operating characteristic (ROC) curves were plotted to further assess the predictive value of different indicators for patients with sepsis / septic shock. RESULTS A total of 295 strains of pathogenic bacteria were cultured and isolated from 191 patients with sepsis/septic shock, of which 212 strains of Gram negative bacteria accounted for 71.86%, 50 strains of Gram positive bacteria accounted for 16.95%, and 32 strains of fungi accounted for 16.75%. The main infection sites were the lungs, abdominal cavity, and skin soft tissues. The overall attainment rate of the 1-hour bundle strategy was 9.95%, with no statistically significant difference between the two groups. The 28 day mortality rate was 47.64%. Age (AUC=0.620), sepsis related sequential organ failure score (SOFA) (AUC=0.730), acute physiology and chronic health Ⅱ(APACHEⅡ) score (AUC=0.662), 6-hour lactate (AUC=0.590), and 24-hour lactate (AUC=0.681) were influencing factors for the 28 day prognosis of sepsis/septic shock patients. The results of Receiver operating characteristic showed that age, SOFA score, APACHE Ⅱ score, 6 h lactate, 24 h lactate had certain predictive value for the prognosis of patients (P<0.05). CONCLUSION The rate of compliance with 1 h bundle strategies in ICUs in this region was not satisfactory and effective strategies should be explored to improve the implementation of evidence-based guidelines, and thereby effectively reduce the threat of sepsis.