Abstract: OBJECTIVE To analyze the relationship between the use of antibiotics and the dynamic tendency of drug resistance rates of common gram-negative bacteria so as to provide guidance for reasonable clinical use of antibiotics.METHODSFrom Jan 2011 to Dec 2016, the DDDs of antibiotics and drug resistance rate of gram-negative bacteria were retrospectively analyzed, and the correlation between the trend of drug resistance of the gram-negative bacteria and the DDDs of antibiotics was observed.RESULTSThe drug resistance rates were increased with the increase of DDDs of antibiotics, and the drug resistance rates of the gram-negative bacteria were increased with the increase of DDDs of cephalosporins ceftazidime and quinolones levofloxacin, the drug resistance rate of Acinetobacter baumannii strains to carbapenems was about 65%, and the drug resistance rates of Escherichia coli strains to ceftazidime and levofloxacin were higher than those of Klebsiella pneumoniae strains.CONCLUSIONThe DDDs of antibiotics is associated with the drug resistance rates of pathogens. It is necessary to strengthen the reasonable use of antibiotics so as to curb the rapid increase of drug resistance rates of the gram-negative bacteria and reduce multidrug-resistant bacteria.