Investigation of blaKPC and blaNDM of carbapenem-resistant Enterobacteriaceae

OBJECTIVE To detect blaKPCand blaNDM of carbapenem-resistant Enterobacteriaceae,and investigate the mechanism of carbapenem-resistance in Enterobacteriaceae.METHODS A total of 27 strains of carbapenem-resistant Enterobacteriaceae from Jan.2014 to Jun.2015 were collected.Enterobacteriaceae bacteria which were resistant to imipenem or meropenem or both of them were screened.Modified Hodge test was used for early screening,and polymerase chain reaction（PCR）amplification was used to detect drug-resistant genes sequencing of producing KPC and NDM.RESULTS A total of 5strains from 27 isolated strains were positive in modified Hodge test,and there were 9strains carrying blaKPCcarbapenem-resistant gene and 13 strains carrying blaNDM carbapenem-resistant gene were detected by PCR.Genetic sequencing showed that KPC-2type carbon penicillium enzyme and NDM-1type resistant genes were related to drug-resistance.CONCLUSION The mechanism of carbapenem-resistance in Enterobacteriaceaeis mainly due to KPC-2type carbon penicillium enzyme and NDM-1type resistant genes,which should be concerned in the clinical and laboratory.